Antibody Heavy Chain Molecular Weight IgG antibodies consist of four polypeptide chains IgG antibodies are large molecules having a molecular weight of approximately 150 kDa composed of two different kinds of polypeptide chain One of approximately 50 kDa is termed the heavy or H chain and the other of 25 kDa is termed the light or L chain Fig 3 2
The type of heavy chain defines the overall class or isotype of an antibody There are five types of mammalian Ig heavy chains denoted by Greek letters and These chains are found in IgA IgD IgE IgG and IgM antibodies respectively A single antibody molecule contains either light chains or light chains but never both Each heavy chain has a molecular weight of 50 000 daltons and consists of a constant and variable region
Antibody Heavy Chain Molecular Weight
Antibody Heavy Chain Molecular Weight
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Each half is made up of a heavy chain of approximately 50 kDa and a light chain of approximately 25 kDa joined together by a disulfide bond near the carboxyl terminus of the light chain There is a folding of polypeptide chains bringing the CDR portion of heavy and light chain in close proximity to Epitope and creates a three dimensional structure that fits the epitope Epitope and paratope are just like lock and key
Each H chain is paired with a L chain The heavy chain components are CH1 CH2 CH3 hinge and the VH and light chains consist of CL and the or chains The approximate molecular weight of an IgG antibody is 150 kDa IgG antibodies have 4 Antibody molecules have a common structure of four peptide chains This structure consists of two identical light L chain polypeptide of about 22000 Da and two identical heavy H chain of larger polypeptide of about 55000 Da or more
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Molecular weight of heavy chains is 50 000 to 70 000 depending upon antibody isotype class Basically an antibody molecule has two functions i e antigen binding and effector functions The terms heavy and light refer to the molecular weight heavy chains have a molecular weight of about 50 000 and light chains have a molecular weight of about 25 000 All antibody or immunoglobulin molecules consist of one or more basic monomer molecule of
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IgG antibodies consist of four polypeptide chains IgG antibodies are large molecules having a molecular weight of approximately 150 kDa composed of two different kinds of polypeptide chain One of approximately 50 kDa is termed the heavy or H chain and the other of 25 kDa is termed the light or L chain Fig 3 2
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The type of heavy chain defines the overall class or isotype of an antibody There are five types of mammalian Ig heavy chains denoted by Greek letters and These chains are found in IgA IgD IgE IgG and IgM antibodies respectively
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